Anti–EHMT1 polyclonal antibody

Catalog #

25220

$335 *

Size: 100 µl

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Polyclonal antibody raised in rabbit against mouse Ehmt1 (euchromatic histone–lysine N–methyltransferase 1), using three KLH–conjugated synthetic peptides containing a sequence from the central part of the protein

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Synonyms

GLP1, KMT1D

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Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Whole serum

Format

aqueous solution

Formulation

Whole antiserum from rabbit containing 0.05% azide

Concentration

100 µl

Immunogen

synthetic peptide

Background

EHMT1 (UniProt/Swiss–Prot entry Q9H9B1) is a histone methyltransferase which specifically methylates histone H3 on lysine 9. H3K9 methylation represents a marker for epigenetic transcriptional repression. This repression is established by recruiting HP1 proteins to the methylated histone. H3K9 methylation may contribute to silencing of MYC and E2F responsive genes and plays a role in murine embryogenesis.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse EHMT1 (Cat. No. 25220). The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:10,600.

Western blot analysis using the antibody directed against EHMT1
Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the antibody against EHMT1 (Cat. No. 25220) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 138 kDa) is indicated on the right; the marker (in kDa) is shown on the left.

Applications

ELISA (1:100 – 1:200)
WB (1:500)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human, mouse

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–EHMT1 polyclonal antibody

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