Anti-HA-Tag, Monoclonal
Catalog #
25010
$275
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Description
Affinity purified anti-HA-tag clone HA.C5/C10, mouse IgG3. Recognizes the N-terminal, C-terminal or internal HA- tagged fusion proteins. Applications: Western Blot (1:1000), Dot Blot, ELISA, Immunocytochemistry (1:200), Immunoprecipitation (1:200), In Situ Hybridisation (1:500-2000). Optimal dilutions/concentrations should be determined by the end user.
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Synonyms
Antibody, HA-tag
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Product Data Gallery
Product Info
Storage and Usage
Citations
Isotype
IgG3
Clonality
clone HA.C5/C10
Host Species/Expression System
Mouse
Purification
Affinity chromatography
Format
Aqueous buffer solution
Formulation
1 mg/ml in 10 mM PBS, pH 7.2, with 0.05% NaN3
Immunogen
Synthetic peptide from influenza hemagglutinin epitope: YPYDVPDYA conjugated to KLH.
Background
Hemagglutinin is an antigenic glycoprotein found on the surface of the influenza viruses, as well as many other bacteria and viruses. It is responsible for binding the virus to the cell that is being infected. The name hemagglutinin comes from the protein's ability to cause red blood cells (erythrocytes) to agglutinate in vitro. The HA tag is derived from the HA molecule corresponding to a.a. 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
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