Anti-GST-Tag, Monoclonal

Catalog #

25009

$275 *

Size: 100 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Affinity purified monoclonal Anti-GST-Tag, clone GST.B6, mouse IgG2a. Recognizes native and denatured forms of purified GST or GST fusion proteins. Applications: Western Blot (1:1000-3000), Dot Blot, ELISA, Immunocytochemistry (1:500-2000), Immunoprecipitation. Optimal dilutions/concentrations should be determined by the end user.

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Synonyms

Antibody, GST-tag

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Product Data Gallery

Product Info

Storage and Usage

Citations

Isotype

IgG2a

Clonality

clone GST.B6

Host Species/Expression System

Mouse

Purification

Protein A Affinity Chromatography

Format

Aqueous buffer solution

Formulation

1 mg/ml in 10 mM PBS, pH 7.2, with 0.05% NaN₃

Immunogen

Purified Schistosoma japonicum GST fusion protein.

Background

GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

Storage/Stability

Store at –20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.

Applications

WB, ICC, IP, ELISA

Shipping Temperature

-20°C or-80°C

Species Reactivity

Schistosoma japonicum. Recognizes native and denatured forms of purified GST or GST fusion proteins. Predicted molecular weight of Schistosoma japonicum GST: 26 kDa.

Cross Reactivity

Works in human cells; predicted to work in all proteins containing the GST tag, regardless of species. Does not cross-react with mammalian GST.

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Antibodies

Regulatory Status

Research Use Only