Human Stromal Cell-Derived Factor-1 alpha Recombinant

Catalog #

90237-B

$205 *

Size: 10 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Recombinant SDF-1 alpha is a disulfide-linked monomer protein consisting of 69 amino acid residues, migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Stromal Cell-Derived Factor-1 alpha was expressed in E. coli.

●

Synonyms

Stromal Cell-Derived Factor-1 alpha, SDF1a, CXCL12, TLSF-A, SCDF-1

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥97% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from a 0.2 µm filtered concentrated (1 mg/ml) solution in 20 mM phosphate buffer, pH 7.2.

8 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

22–89

Biological Activity

Determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentration range of 10-40 ng/ml.

Genbank #

P48061

UniProt #

P48061

Background

SDF-1alpha and SDF-1beta are small cytokines belonging to the CXC- Chemokines. SDF-1 is identical with a chemokine reported to function as a pre-B-cell growth factor in the presence of IL-7 and isolated originally from a murine bone marrow stromal cell line. Human SDF-1alpha and SDF-1beta are encoded by a single gene and arise by alternative splicing. SDF-1 acts on lymphocytes and monocytes but not neutrophils in vitro and is a highly potent chemoattractant for mononuclear cells in vivo. In addition, SDF-1 also induces intracellular actin polymerization in lymphocytes. SDF acts as a chemoattractant for human hematopoietic progenitor cells expressing CD34 giving rise to mixed types of progenitors, and more primitive types. The chemotactic response is inhibited by pertussis toxin. Chemotaxis of CD34(+) cells in response to SDF is increased by IL-3 in vitro. SDF has been shown also to induce a transient elevation of cytoplasmic calcium in these cells.

References

1. Hiesinger W, et al. Trends Cardiovasc Med. 2012 Aug,22(6):139-44.
2. Felix AS, et al. Cancer Microenviron. 2010 Feb 26,3(1):49-56.

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Human Stromal Cell-Derived Factor-1 alpha Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY