Human Stem Cell Factor Recombinant

Catalog #

90235-B

$270 *

Size: 10 µg

Qty

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Description

Recombinant SCF (Kit Ligand) is a disulfide-linked monomer protein consisting of 165 amino acid residues, and migrates as an approximately 18 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human SCF mature chain was expressed in E. coli.

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Synonyms

Kit Ligand, SCF, Stem Cell Factor, Steel Factor, MGF, and Mast Cell Growth Factor, KITL, CD117L

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥97% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from a 0.2 µm filtered PBS solution, pH 7.0.

18 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

26–189

Biological Activity

The ED₅₀ was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 10⁵ units/mg.

Genbank #

P21583

UniProt #

P21583

Background

SCF is a stromal cell-derived cytokine synthesized by fibroblasts and other cell types. SCF promotes proliferation and early differentiation of cells at the level of multipotential stem cells. It has been suggested that SCF is essential for optimal production of various hematopoietic lineages, mainly because of its ability to prevent apoptosis when it co-stimulates with other cytokines. The receptor for SCF, designated SCFR(CD117), is the oncogene designated as KIT. The biological activities of SCF are synergised considerably by colony stimulating factors GM-CSF and G-CSF, and also by IL-7, Epo and some other growth and differentiation factors. In combination with IL-7, SCF stimulates the proliferation of pre-B-cells. SCF is also a potent chemoattractant for cells, for example, mast cells, expressing the kit receptor. One response to SCF in these cells is a characteristic rearrangement of the actin filaments of the cytoskeleton.

References

1. Bedell MA, et al. J Androl. 2004 Mar-Apr,25(2):188-99.
2. Heissig B, et al. Thromb Haemost. 2003 Oct,90(4):570-6.
3. Wehrle-Haller B. Pigment Cell Res. 2003 Jun,16(3):287-96.

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Human Stem Cell Factor Recombinant

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