TCF/LEF Luciferase Reporter Lentivirus (Wnt/β-catenin Signaling Pathway)

Catalog #
79787
$875 *
Size: 500 µl x 2
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Description

The TCF/LEF Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene under the control of TCF/LEF-responsive element located upstream of the minimal TATA promoter (Figure 1). After transduction, activation of the Wnt/β-catenin signaling pathway in the target cells can be monitored by measuring the luciferase activity.

Figure 1. Schematic of the lenti-vector used to generate the TCF/LEF luciferase reporter lentivirus

This product has been cited 5 times.

Synonyms
TCF reporter. LEF reporter, lentivirus reporter, Wnt reporter, β-catenin pathway
Product Info
Storage and Usage
Citations5
Supplied As
Two vials (500 μl x 2) of TCF/LEF luciferase reporter lentivirus at a titer ≥1 x 10^7 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Materials Required But Not Supplied

• LiCl (Sigma, #L7026)
• Mouse Wnt3a (R&D Systems 1324-WN)
• HEK293 growth medium or use Thaw Medium 9 (BPS Bioscience, #79665): MEM with
10% FBS, 0.1 mM nonessential amino acids, 1 mM sodium pyruvate
• Polybrene (Millipore, #TR-1003-G)
• 96-well tissue culture treated white clear-bottom assay plate (Corning, #3610)
One-Step luciferase assay system (BPS Bioscience, #60690)
• Luminometer

Formulation
The lentiviruses were produced from HEK293T cells and are provided in medium containing 90% DMEM + 10% FBS.
References

1. Tian S., et al., Blood. 1994; 84(6):1760-1764.
2. Zhong, Z., et al., Science. 1994; 264(5155):95-98.