TCF/LEF Reporter Kit (Wnt Signaling Pathway)
The TCF/LEF Reporter kit is designed for monitoring the activity of Wnt / β-catenin signaling pathway in the cultured cells. The kit contains transfection-ready TCF/LEF luciferase reporter vector, which is a Wnt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.
For assays in more difficult to transfect cell types, we suggest using our lentiviral products. The corresponding lentiviral product for this kit is BPS Bioscience #79787.
This product has been cited 31 times.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector
• Mammalian cell line and appropriate cell culture medium
• 96-well tissue culture plate or 96-well tissue culture treated white clear-bottom assay plate (Corning # 3610)
• Transfection reagent for mammalian cell line [We use Lipofectamine™ 2000 (Invitrogen # 11668027). However, other transfection reagents work equally well.
• Opti-MEM I Reduced Serum Medium (Invitrogen #31985-062)
•TWO-Step Luciferase (Firefly & Renilla) Assay System:
TWO-Step Luciferase (Firefly & Renilla) Assay System (BPS Bioscience, #60683): This system assays cells directly in growth medium. It can be used with any luminometer. Automated injectors are not required.
• Luminometer
Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)
1. Clevers H (2006) Wnt/beta-catenin signaling in development and disease. Cell 127(3):469-480.
2. Chen B et al. (2009) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chemical Biology 5(2):100-107.
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