TCF/LEF StemBright™ Luciferase iPS Cell Pool (Wnt Pathway)

Catalog #

78515

$5,950 *

Size: 1 vial

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The TCF/LEF (T-cell factor/lymphoid enhancing factor) StemBright™ Luciferase Cells contain a firefly luciferase gene under the control of TCF/LEF responsive elements, stably integrated into induced Pluripotent Stem (iPS) cells. TCF/LEF transcription factors are downstream of the Wnt signaling pathway. This cell pool is validated for its response to GSK3β inhibitor CHIR-99021, which activates the Wnt signaling pathway in human pluripotent stem cells.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

iPSC

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Product Data Gallery

Immunofluorescence staining of TCF/LEF StemBright™ Luciferase iPS Cell Pool (Wnt Pathway) for Oct-4

Dose response of GSK3-β inhibitor CHIR-99021 in TCF/LEF StemBright™ Luciferase iPS Cell Pool

Product Info

Storage and Usage

Citations

Host Cell Line

Non-Disease Human iPS Cell Line (iXCells 30HU-002)

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of iPS cell freezing medium

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
mTeSR™ Plus	Stem Cell Technologies #100-0276
Matrigel™	Corning #354230
DMEM/F12	Thermo Fisher #11330032
Thiazovivin	BPS Bioscience #78506
RelesR	Stem Cell Technologies #05872
Accutase	Thermo Fisher #A1110501

Materials Used in the Cellular Assay

Name	Ordering Information
CHIR-99021	BPS Bioscience #27614
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

The Wnt signaling pathway is a well described regulator of organism development and cell fate specification. Cellular pluripotency and differentiation of multiple lineages, including cardiomyocytes and neurons are modulated by this pathway. There are 19 Wnt ligands and 10 Frizzled receptors, which stimulate canonical as well as non-canonical Wnt pathways. In the canonical pathway, cell activation by a Wnt ligand leads to the stabilization and nuclear translocation of β-catenin and subsequent activation of TCF/LEF transcription factor. The Wnt signaling pathway has recently been shown to play a role in cell cycle reentry and proliferation of iPS-derived cardiomyocytes. It is a therapeutic target in the treatment of neuropsychiatric disorders.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

As this is a cell pool and not a cell line, BPS Bioscience cannot guarantee the stability of the genetic modifications over time. Clonal derivation may occur. We recommend freezing cell vials very early on and growing the cells for a limited number of passages. Cells should be cultured using Growth Media, which contain selection antibiotics.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture

iPSC Thaw Medium: mTeSR™ Plus supplemented with 1% Penicillin/Streptomycin

Complete iPSC Thaw Medium: mTeSR™ Plus supplemented with 1% Penicillin/Streptomycin and 1 µM Thiazovivin.

Thiazovivin is unstable once diluted in the medium. Prepare just enough to proceed with thawing the cells.

iPSC Growth Medium: mTeSR™ Plus supplemented with 1% Penicillin/Streptomycin and 0.5 µg/ml Puromycin.

iPSC Passage Medium: mTeSR™ Plus supplemented with 1% Penicillin/Streptomycin and 0.5 µg/ml Puromycin and 1 µM Thiazovivin.

2X Freezing Medium: 80% mTeSR™ Plus supplemented with 1% Penicillin/Streptomycin, 1 µM Thiazovivin and 20% DMSO (vol/vol).

Media Used in the Functional Cellular Assay

iPSC Thaw Medium: mTeSR™ Plus supplemented with 1% Penicillin/Streptomycin (does NOT contain Thiazovivin).

Applications

Screen for Wnt-activating small molecules in iPS cells

Shipping Temperature

-80°C

Notes

License Disclosure
The iPSC technology is protected by several patents, including US patent Nos. 8048999, 8058065, 8129187, 8278104, 8530238, 8900871, 9404124, 9499797, 10519425, and patent pending, for which iPS Academia Japan, Inc. has been granted license rights with a sub-licensable right.

The purchase of this cell line grants you a 10-year license to use this cell line in your immediate laboratory for internal research purposes. Commercial use of this cell line is not allowed. Commercial use requires the appropriate license from iPS Academia Japan, Inc.

This cell line is for research use only, not for therapeutic or prophylactic use in humans or animals. Use in humans is strictly prohibited.

This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of the cell line in any way. Modification of this cell line or transfer to another facility of the cells requires a separate license or additional fees; contact [email protected] for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use and does not accept any liability in connection with the handling or use of the cell line.

Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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TCF/LEF StemBright™ Luciferase iPS Cell Pool (Wnt Pathway)

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