SARS-CoV-2 IgG Detection Kit (Colorimetric Anti-Spike S1 RBD IgG detection)
The SARS-CoV-2 IgG detection kit is designed for qualitative detection of human IgG antibodies in serum collected from individuals suspected of prior infection with the virus that causes COVID-19. This fast and simple ELISA uses the receptor binding domain (RBD) of the SARS-CoV-2 Spike protein (BPS Bioscience #100696) to identify IgG antibodies that indicate a previous infection with SARS-CoV-2.
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PBS (Phosphate buffered saline)
PBST (Phosphate buffered saline containing 0.05% Tween-20)
Dry Milk (Fisher #115668 or compatible)
1N HCl (aqueous)
Rotating or rocker platform
UV/Vis spectrophotometer microplate reader capable of reading absorbance at 450 nm
Catalog Number |
Component |
Amount |
Storage |
|
100696 | Spike S1 RBD, Avi-His tag (SARS-CoV-2) | 5 µg | -80°C | Avoid multiple freeze/ thaw cycles! |
anti-human Fc-HRP conjugate (1 mg/ml) | 5 µl | +4°C | ||
Colorimetric HRP substrate | 10 ml | +4°C | ||
79964 | Transparent 96-well microplate | 1 | Room Temp |
*The initial concentration of Spike RBD is lot-specific and will be indicated on the tube containing the protein
The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein recognizes and binds to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection.
Long, Q.-X., et al. 2020. Antibody responses to SARS-CoV-2 in patients with COVID-19. Nat. Med. (in press). https://doi.org/10.1038/s41591-020-0897-1