PKCθ (PKCtheta), GST-tag Recombinant

Catalog #

40165

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human PKCθ (PKCtheta), full length, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.

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Synonyms

PRKCQ, PRKCT, MGC126514, MGC141919, nPKC-theta, PKCθ protein kinase c theta

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥75%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

110 kDa

Amino Acids

full length

Genbank #

NM_006257

UniProt #

Q04759

Tag(s)

N-terminal GST-tag

Background

Protein Kinase C theta (PKCθ) is important component in the intracellular signaling cascade (1). Recent studies have suggested that local accumulation of fat metabolites inside skeletal muscle may activate a serine kinase cascade involving PKCθ leading to defects in insulin signaling and glucose transport in skeletal muscle (2). Insulin resistance plays a primary role in the development of type 2 diabetes and may be related to alterations in fat metabolism. PKCθ is a crucial component mediating fat-induced insulin resistance in skeletal muscle and is a potential therapeutic target for the treatment of type 2 diabetes (2).

References

1. Manicassamy, S. and Sun, Z. The critical role of protein kinase C-theta in Fas/Fas ligand-mediated apoptosis. J. Immunol. 2007;178(1):312-9.
2. Kim, J K. et al: PKC-theta knockout mice are protected from fat-induced insulin resistance. J. Clin. Invest. 2004;114(6):823-7.

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PKCθ (PKCtheta), GST-tag Recombinant

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