LCK Assay Kit

Catalog #

79794

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The LCK Assay Kit is designed to measure LCK activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

Need us to run inhibitor screens or profile your compounds against LCK? Check out our Kinase Screening Services.

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Synonyms

LCK, Leukocyte C-Terminal Src Kinase, T-Lymphocyte Specific Protein Tyrosine Kinase, lck

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The LCK Assay Kit comes in a convenient 96-well format, with enough purified recombinant LCK, Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1), ATP, and kinase assay buffer for 96 enzyme reactions.

Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 0.5 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog Number	Reagent	Amount	Storage
40470	LCK, GST-Tag	5 µg	-80°C	Avoid multiple freeze/thaw cycles!
79793	5x Kinase Buffer 2	1.5 ml	-20°C
79686	ATP (500 μM)	100 µl	-20°C
40217	Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1) (10 mg/ml)	100 µl	-20°C
79696	96-well plate, white	1	RT

UniProt #

P06239

Background

LCK is a tyrosine kinase that phosphorylates the CD3 receptor and is essential for T cell development and activation. LCK also associates with the cytoplasmic domains of the CD4 and CD8 glycoproteins and interacts with the beta-chain of the interleukin-2 receptor. LCK inhibitors are being investigated as a promising therapeutic approach to autoimmune disease and other inflammatory disorders.

References

1. Yu, C.-L., Jove, R., and Burakoff, S.J. 1997. "Constitutive activation of the Janus kinase-STAT pathway in T lymphoma overexpressing the LCK protein tyrosine kinase." J. Immunology 159 (11): 5206-5210.

2. Veillette, A., et al. 1989. "Signal transduction through the CD4 receptor involves the activation of the internal membrane tyrosine-protein kinase p56LCK." Nature 338(6212): 257-259.