LCK, GST-tag Recombinant

Catalog #

40470

$365 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human LCK, also known as Leukocyte C-terminal Src kinase, Lymphocyte cell-specific protein-tyrosine kinase, IMD22, and LSK, GenBank Accession No. NM_005356, a.a. 2- 509(end), with N-terminal GST-tag, expressed in Sf9 insect cells using a baculovirus expression system. MW=85 kDa.

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Synonyms

LCK, Leukocyte C-Terminal Src Kinase, T-Lymphocyte Specific Protein Tyrosine Kinase, lck

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, 1.6 mM glutathione.

85 kDa

Amino Acids

2-509(end)

Specific Activity

≥200 pmol/min/μg

Genbank #

NM_005356

UniProt #

P06239

Tag(s)

N-terminal GST-tag

References

1. Fischer, S. et al. Biochem Biophys Res Commun. 1987 Mar 30,143(3):819-26.
2. Veillette, A. et al. Oncogene Res. 1987 Sep-Oct,1(4):357-74.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was done in Kinase buffer containing 5 mM MnCl₂, 1 mM DTT using Poly-(Glu:Tyr) substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Cytoplasmic Tyr

Regulatory Status

Research Use Only

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Datasheets/Protocols

40470 Lot 170801-B2.pdf

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