FcRn Complex (FCGRT/B2M), His-Avi-Tag, Biotin-Labeled, HiP™ Recombinant

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Catalog #

71283

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Description

Recombinant human FcRn complex composed of FcRn [also known as Neonatal Fc receptor or FCGRT (Fc Fragment of IgG Receptor and Transporter)], encompassing amino acid 24-297 and B2M (beta 2 microglobulin), encompassing amino acids 21-119. FCGRT has a C-terminal His-tag (10xHis) followed by an Avi-tag™. This protein is enzymatically biotinylated using Avi-Tag™ technology. These proteins are co-expressed in a HEK293 expression system and affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration.

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Biotin-Avidin Pulldown

Gel Filtration Curve

Fc (IgG1): FcRn inhibition curve using a colorimetric assay (#78501). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

FCGRT (24-297-His-Avi)-(Biotin) / B2M (21-119)

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

FCGRT: 34 kDa; B2M: 14 kDa + glycans

Amino Acids

FCGRT: 24-297; B2M: 21-119

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Aggregation

<10%

Genbank #

FCGRT: NM_001136019; B2M: NM_004048

UniProt #

FCGRT: P55899; B2M: P61769

Tag(s)

C-terminal His-Avi-tag

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

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Datasheets/Protocols

71283 Lot 220908

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FcRn Complex (FCGRT/B2M), His-Avi-Tag, Biotin-Labeled, HiP™ Recombinant

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