CDK2 (no tag)/CyclinA2, His-GST-tags Recombinant

Catalog #

41101

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Complex of CDK2 (cyclin dependent kinase 2) and cyclin A2 (CCNA2).

Recombinant human CDK2, full length encompassing amino acids 1-298 (end), does not contain a tag. Recombinant human Cyclin A2, encompassing amino acids 174-432 (end), contains an N-terminal His-tag (6xHis) followed by a GST-Tag. The two proteins were co-expressed in Sf9 insect cells and spontaneously formed a complex, which was affinity purified.

This product has been cited 1 time.

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Synonyms

CDKN2, Cell division protein kinase 2, p33 protein kinase, p33(CDK2), CCN1, CCNA, cyclin A, cyclin-A2, CDK-2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations1

Species

Human

Construct

CDK2 (1-298(end) / CyclinA2 (His-GST-174-432(end))

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

20 mM NaH₂PO₄/Na₂HPO₄, pH 8.0, 400 mM NaCl, 10% glycerol, 2 mM DTT, and 1 mM EDTA

CDK2: 34 kDa; CyclinA2: 57 kDa

Amino Acids

CDK2 a.a. 1–298 (end), CyclinA2 a.a. 174–432 (end)

Specific Activity

203 pmol/min/µg

Genbank #

CDK2: NM_001798; CyclinA2: NM_001237

UniProt #

P24941 (CDK2), P20248 (CyclinA2)

Tag(s)

CDK2 no tag, CyclinA2 N-terminal His-GST-tags

References

1. Brown NR., et al., Cell Cycle. 2007 Jun 1,6(11):1350-9.
2. Hara KT., et al., Dev Biol. 2005 Oct 1,286(1):102-13.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was done according to the CDK2 Assay Kit (BPS Bioscience #79599) with various amounts of CDK2/CyclinA2.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

41101 Lot 211208-G

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CDK2 (no tag)/CyclinA2, His-GST-tags Recombinant

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