CDK1/CyclinA2, GST-Tag Recombinant
Catalog #
40100
$410
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Description
Complex of human CDK1 (Cyclin Dependent Kinase 1) and human Cyclin A2. The two constructs are: recombinant human CDK1, full length encompassing amino acids 2-297 (end) and recombinant human Cyclin A2, full length encompassing amino acids 2-432 (end). Both constructs contain an N-terminal GST-Tag. A short peptide linker placed between GST and the protein includes a PreScission protease cleavage site. The two proteins were co-expressed in Sf9 insect cells and spontaneously formed a complex, which was affinity purified.
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Synonyms
CDK1: Cyclin-dependent kinase 1, CDK-1, Cell division protein kinase 1, cell division control protein 2 homolog, CDC2, CDC28A, p34 protein kinas, P34CDC2
Cyclin A2: CCNA2, CCN1, CCNA
Cyclin A2: CCNA2, CCN1, CCNA
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Product Data Gallery
Product Info
Storage and Usage
Citations
Species
Human
Construct
CDK1 (GST-Pre-2-297(end)) / CyclinA2 (GST-Pre-2-432(end))
Host Species/Expression System
Coexpressed in Sf9
Purity
≥90%
Format
Aqueous buffer solution
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT
MW
CDK1: 60 kDa; CyclinA2: 75 kDa
Amino Acids
CDK1: 2-297(end); CyclinA2: 2-432(end)
Specific Activity
205 pmol/min/µg
Genbank #
CDK1: NM_001786; CyclinA2: NM_001237
UniProt #
CDK1: P06493; CyclinA2: P20248