Search results for: 'luciferase assay system'

SARS-CoV-2 Variants: Evading the Immune System The COVID-19 pandemic, caused by the novel SARS-CoV-2 coronavirus , has ravaged the globe, infecting over 145 million people and causing over 3 million deaths as of the time of this article. Ending the pandemic will require a combination of effective interventions, including therapeutics, diagnostics, and vaccines.  Several monoclonal antibody therapeutics have received emergency use authorization, and a number of vaccines have been devel...

Cell Signaling Pathway Reporter Systems Cell signaling plays a pivotal role in biology as it regulates various physiological processes crucial for the functioning of organisms. Cell signaling encompasses a complex network of communication pathways that enable cells to perceive and respond to external cues, including hormones, growth factors, and environmental stimuli. Understanding cell signaling pathways is essential fo...

Diabetes & Obesity Breakthrough treatments for diabetes and obesity aim to stem the rising epidemic of complications and deaths directly attributed to these conditions. Diabetes and obesity are two interconnected health conditions that pose significant global challenges. Diabetes is a chronic metabolic disorder characterized by elevated blood sugar levels, resulting from either insufficient insulin production (Type 1 diabetes) or ineffective utili...

New Customer Discount Program Make the most out of your first order with us. Place your first order with us as a new customer and get up to a 50% discount . Some restrictions apply. Terms For new US customers only. 10% discount  on Assay Kits , Lentiviruses , and Cell Lines . 30% discount  on ONE-Step™ Luciferase Assay System ( #60690 ), TWO-Step Luciferase (Firefly & Renilla) Assay System ( #60683 ), Western EC...

Bispecific Antibodies & BiTE® (Bispecific T-Cell Engager) Services   CD3/CD19 BiTE® - Mechanism of Action The CD3/CD19 BiTE® is a biopharmaceutical drug known as a Bi-specific T-cell engager for a second-line treatment for Philadelphia chromosome-negative relapsed or refractory acute lymphoblastic leukemia. It binds to CD19 on cancer cells (e.g. Raji cells) and CD3 on T cells simultaneously, thus bringing T lymphocytes closer to cancer cells expressing specific cancer ...

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Resources Your time is a precious resource. Our content will help you get up to speed quickly and easily with clear and concise information. Learn more about topics in biology and drug discovery, delve into specifics on how best to use our products, or read about how others have applied our products to their research. If you have more questions, our teams are ready to provide support: Contact Us . Technical Notes & Application Notes eBooks Webinars and Video...

Methods For Drug Discovery   Immunotherapies  based on targeting the  PD-1:PD-L1  immune checkpoint pathway are having clinical impact and are giving new hope to cancer patients. However, positive response to treatment is not guaranteed and resistance remains a concern. Improving outcomes based on immunotherapy requires multiple approaches for manipulating the complexities of the immune system and tumor microenvironment. To meet these challenges, a series of r...

Bispecific Antibodies & BiTE® (Bispecific T-Cell Engager) Clinical & Therapeutic Research An antibody (Ab) containing two different antigen-binding sites within one molecule is known as a bispecific antibody (BsAb).  BsAb research has expanded greatly within the past ten years. To date, two BsAbs,  Blinatumomab   and Emicizumab, have been approved in the US and the EU, while a third BsAb, Catumaxomab, is already commercially available in the EU. Additionally, th...

Cell Line Frequently Asked Questions FAQ Search: Custom Cell Line Development Generate stable cell lines for protein overexpression, mutations or gene knock-out. The development process involves pre-defined milestones with data provided at the completion of each milestone. Each project is customized. Learn more How should I store the cells? Cells are shipped in dry ice, and upon receipt should immediately be thawed for culture...

Cell Lines and Primary Cells Cell lines are extremely useful tools for the study of protein interactions, gene activation, cell signaling, viral infection, cell-to-cell communication, and other biological processes in the context of an intact cell system rather than cell-free biochemical assays. BPS Bioscience has developed over 250 stable cell lines applicable across a wide range of research areas including immunotherapy, CAR-T cell therapy, cell signaling, metabolism, neuroscience, and mu...

IgG Antibodies and Fcγ Receptors in Immunotherapy IgG Antibodies and Fcγ Receptors in Immunotherapy Monoclonal, immunoglobulin G (IgG), antibodies are the basis of powerful therapies for treating cancer, viral infections, and autoimmune disease.  The power of therapeutic antibodies comes from their ability to bind antigen targets with high specificity and then harness the functions of the larger the immune system.  There are a variety of immune responses. Cytotoxic anti...

Viral Tools Frequently Asked Questions FAQ Topic: -Any- General Lentiviruses AAVs FAQ Search: How should I store the viral particles (lentivirus, AAV or VSV) that I just rec...

Frequently Asked Questions BPS Bioscience is here to answer your questions and provide project guidance. On the following pages, we outline some of the most frequently asked questions separated by product type. If your question is not answered through these resources, please contact us today and we are happy to assist.  Proteins Cell Lines Assay Kits ...

Citations   This page contains a list of scientific journal articles that have cited BPS products and services since 2013. We would like to congratulate all our loyal customers on their contributions to the scientific community. Our customers are truly amazing and dedicated scientists and this is evidenced by the outstanding research they are doing.  Search By Product Name or Catalog Number Name Title 10X HAT Assay Buffer (50095...

Cell-Based Assays and Expression Kits Cells can be engineered to serve as readouts for cell-based assays as a complement or alternative to biochemical assays. A variety of cell-based assay types can be used for research and development applications, including signaling pathway analysis, inhibitor/activator studies, CAR-T cell functional screening, ADCC assays, high-throughput screening systems and much more. We provide a variety of cell-based assay express...

Cell Signaling Pathway Screening & Profiling In order to determine effectors of cell signaling pathways in the context of intact functioning cells, cell-based reporter assays are an ideal option. Using luciferase reporters downstream of a pathway-specific promoter response element, signaling activity can be conveniently measured by the output of luciferase activity. The system can be applied across a wide variety of cell types and signaling pathways and allows for ...

Cell Cytotoxicity Screening & Profiling Services Small Molecule Cell Cytotoxicity Assays Understanding cell cytotoxicity, either as a result of targeted therapy or as a side effect, is important in drug development. Our expert scientists can help determine effective dose curves for your molecules of interest in whole cell assays, whether they are small molecules or biologics, including antibody drug conjugates. Over 30 different tumor cell lines are availabl...

Small Molecule Cancer Therapy Targets For some cancers, small molecules that disrupt, inhibit, or agonize target protein function may be the best therapeutic option. Indeed, many new targeted therapeutics are now approved together with companion diagnostics to identify patient populations most likely to benefit from the new treatment. For some cancers, small molecules that disrupt, inhibit, or agonize target protein function may be the best therapeutic...

CD19 Research Products CD19, a cell surface protein specific to B cells, has emerged as an effective immunotherapy target to treat B cell malignancies and autoimmune diseases. By leveraging monoclonal antibodies, chimeric antigen receptor (CAR) T cells and NK cells, or bispecific T cell/NK cell engagers (BiTEs/BiKEs), CD19-directed immunotherapies aim to selectively eliminate CD19-expressing cells. In cancer, these ther...

Conferences & Events Development of a Cell-Based Reporter Assay for Measuring ADAR1 Editing Activity for Drug Discovery and Cell Biology Research May 21, 2024 (8:00 AM PT, 11 AM ET) Adenosine Deaminase Acting on RNA (ADAR) enzymes perform adenosine to inosine base editing in RNA, particularly targeting adenosines located within a specific double-stranded stem-loop motif.  ADAR1 plays important roles in inflammation, autoimmune diseases, and cancer, and represents ...

Neuroscience Neuroscience research is making strides towards solutions for CNS-related disorders. By exploring the structure, function, and development of the central nervous system (CNS), neuroscience provides insights into the fundamental mechanisms underlying how our brains function. This knowledge is essential for advancing our comprehension of neurological disorders, mental health conditions, brain malignancies, and brain-related inj...

Adoptive Cell Therapy Using Antigen-Specific TCRs Engineered T cell receptors provide a promising therapeutic approach to fight cancer by focusing immune cells to target specific antigens. Antigen-specific T-cell receptors (TCRs) play a crucial role in the adaptive immune response by recognizing specific antigen peptides presented by MHC molecules on the surface of infected or abnormal cells. These TCRs are typically found on the surface ...

Custom CAR-T Cell Development CAR-T cell therapy is a revolutionary cancer treatment that involves genetically engineering immune cells, using either the patient’s own cells or allogeneic cells, to recognize and attack cancer cells using a Chimeric Antigen Receptor (CAR). This therapy has shown remarkable success in treating some types of blood cancers, such as leukemia and lymphoma, even in patients who have not responded to other treatments. While CAR-...

In Vitro Toxicity Research Solutions In vitro toxicity testing plays a critical role in drug development by enabling early and efficient identification of potential safety concerns, reducing reliance on animal testing, and supporting regulatory requirements for drug approval. In vitro toxicity testing plays a critical role in drug development by enabling early and efficient identification of potential safety concern...

Lentiviruses for SARS-CoV-2 Research To understand the mechanism of SARS-CoV-2 cell entry, it is essential to study how Spike proteins interact with the ACE2 receptor. However, such studies are hampered by the danger of producing and manipulating live coronavirus. A safer alternative to using live coronavirus is to use recombinant lentivirus pseudotyped with the coronavirus S protein. The Spike pseudotyped lentivirus includes a luciferase reporter, allowing the monitoring of viral entry into...

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HVEM, LIGHT & BTLA: New Immunotherapy Target The checkpoint receptors  HVEM ,  LIGHT ,  CD160 , and  BTLA  are part of a complex network of overlapping receptor interactions that function in both immune stimulation and suppression 1 ,  2  .  This regulatory function has made them therapeutic targets for treatment of cancer, autoimmune diseases and allergies, and for improved methods of organ transplants.  While presenting opportunities for...

The Promise of NK Cell Therapeutics Introduction NK cells are the first line of defense against cancer and have been in the spotlight as a cancer therapy in the last decade. Their advantages versus T cells, such as their safety profile and not requiring MHC (Major Histocompatibility Complex)-antigen stimulation to become cytotoxic, have made NK cells attractive. However, as with T cells, the TME (tumor microenvironment) can suppress their activity, and infiltration into solid tumors is s...

iPSC-Derived Cells Induced pluripotent stem cells (iPSC), pioneered by Nobel Prize winner Shinya Yamanaka, are generated by converting mature human somatic cells via expression of four critical transcription factors known as Yamanaka factors: Myc, Sox2, Klf4, and Oct3/4. iPSCs are an important tool for research because they are capable of differentiating into various cell types, including those of different tissues and organs. They can be used to study disease mechanisms, drug developm...

Research Applications for Engineered iPS Cells Introduction New avenues of research were dramatically opened by the discovery of Takahashi and Yamanaka in 2006 that the ectopic expression of Oct4 (octamer-binding transcription factor 4), Sox2 (SRY-box2), Kfl4 (Kruppel-like factor 4) and c-Myc was sufficient to reprogram terminally differentiated cells into induced pluripotent stem (iPS) cells. These have infinite expansion potential and similar genetic markers, epigenetics and multilineag...