WWP1, FLAG-tag Recombinant

Catalog #

80405

$370 *

Size: 20 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Recombinant human WWP1 (WW Domain Containing E3 Ubiquitin Protein Ligase 1), full length, encompassing amino acids 2-end. This construct contains an N-terminal FLAG-tag. This protein was affinity purified.

●

Synonyms

WW Domain Containing E3 Ubiquitin Protein Ligase 1, WWP1, Atrophin-1 Interacting Protein 5, AIP5, Tiul1

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Inhibition of WWP1 auto-ubiquitination by Methylated Ubiquitin. *Not all lots have been tested for enzyme activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

WWP1 (FLAG-2-922(end))

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, variable FLAG peptide, 10% glycerol, and 3 mM DTT.

106 kDa

Amino Acids

2-922(end)

Genbank #

NM_007013

UniProt #

Q9H0M0

Tag(s)

N-terminal FLAG-Tag

Background

WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1) is a HECT-type E3 Ub ligase belonging to the NEDD4 family of E3 ligases. WWP1 interacts with and ubiquitinates many substrates, including transcription factor ΔNp63 as well as phosphatase and tensin homolog (PTEN), and regulates the degradation of sodium channels and membrane receptors. Genetic mutations or regulatory defects involving WWP1 are associated with neurological disorders and cancer. Aberrant expression of WWP1 in gastric, prostate, and breast cancer, for instance, is an area of high interest, and therefore, WWP1 represents an excellent candidate for therapeutic targeting in multiple cancer types.

References

1. Li Y. et al., Genome Res. Oncogene 28 (33), 2948-2958 (2009).
2. Chen C. Et al., Int. J. Cancer 124 (12), 2829-2836 (2009).

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was performed using the WWP1 Intrachain TR-FRET Assay Kit (BPS Bioscience #78554) with WWP1 protein titrated at various concentrations.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitin E3 ligase

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

80405 Lot 220520

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

●

WWP1, FLAG-tag Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY