PRMT5/MEP50 Assay Service
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Target
PRMT5/MEP50
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Description
Screening and/or profiling inhibitor compounds against PRMT5/MEP50 methyltransferase activity in a biochemical assay.
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Synonyms
PRMT5, HRMT1L5, IBP72, JBP1, SKB1
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Example Data
*Example only, final data may vary.
Assay Details
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Assay Format
Chemiluminescent
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Reference Compounds and IC50
EPZ015666, 0.069 μM
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Assay Principle
The PRMT5/MEP50 Chemiluminescent Assay is designed to measure PRMT5/MEP50 activity for screening and profiling applications. The key to the assay is a highly specific antibody that recognizes methylated R3 residue of Histone H4. In this assay, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, a primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence which is then measured using a chemiluminescence reader.
Target Details
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Protein Family
Methyltransferases
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UniProt
O14744
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Background
PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3.
Delivery
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Estimated Turnaround
Two to three weeks following delivery of compounds
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Results
Extensive report with raw and analyzed data, graphs, and detailed protocols. Includes positive control for inhibition.
