PIKFYVE, GST-Tag Recombinant

Catalog #

101323

$465 *

Size: 10 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Recombinant human PIKFYVE (phosphoinositide kinase, FYVE-type zinc finger containing, also known as PIP5K3), encompassing amino acids 1400-2089(end). The construct contains an N-terminal GST-tag. The protein kinase was affinity purified and is kinase-active.

●

Synonyms

1-phosphatidylinositol 3-phosphate 5-kinase, PIP5K3, PIPkin-III, FYVE-type zinc finger containing phosphoinositide kinase, PIKFYVE, KIAA0981, CFD, FAB1, HEL37, PIP5K, ZFYVE29

●

Product Data Gallery

4-20% SDS-Page Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

PIKFYVE (GST-1400-2098(end))

Host Species/Expression System

Sf9

Purity

85%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol

110 kDa

Amino Acids

1400-2098(end)

Specific Activity

110 pmol/min/µg

Genbank #

NM_015040

UniProt #

Q9Y2I7

Tag(s)

N-terminal GST-tag

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

PIKFYVE activity was measured using the ADP-Glo™ Kinase Assay kit (Promega; Cat# V9101) which quantifies the amount of ADP produced by phosphorylation. The ADP-Glo™ Reagent is added to terminate the reaction and quench the remaining ATP. The Kinase Detection Reagent is then added to convert ADP to ATP and to measure the newly converted ATP using a luciferase reaction.

Assay: kinase activity was measured using a PIP3:PS substrate (250:2000 µM stock solution, sonicated for 1 min before use). The reaction was initiated by mixing Increasing amounts of the protein kinase with 50 µM ATP (final concentration) in 45 mM HEPES, pH 7.5, 40 mM NaCl, 1.6 mM MgCl₂, 0.5 mM EGTA, 20 µg/ml BSA, 40 µM fresh DTT, 0.03% Trition-X100 and PIP3:PS substrate (50:400 µM final). The reaction was terminated by addition of an equal volume of the ADP-Glo™ Reagent supplemented with 10mM MgCl₂, and the Kinase Detection Reagent was added. Phosphorylation was measured by detection of luminescence. The blank was determined from a “no kinase” sample.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Kinase/Lipids

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

101323 Lot 240111

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

●

PIKFYVE, GST-Tag Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY