PRMT5 Chemiluminescent Assay Kit
The PRMT5 Direct Activity Chemiluminescent Assay Kit is designed to measure PRMT5 activity for screening and profiling applications. The key to the PRMT5 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Need us to run inhibitor screens or profile your compounds against PRMT5? Check out our Methyltransferase Screening Services or DNA Replication & Repair Screening Services.
Catalog Number |
Components | Amount | Storage | |
51045 | PRMT5/MEP50 human enzyme | 20 µg | -80°C | Avoid freeze/ thaw cycles! |
52120 | 100 µM S-adenosylmethionine | 250 µl | -80°C | |
52150-3 | Primary antibody 4-3 | 100 µl | -80°C | |
52131H | Secondary HRP-labeled antibody 2 | 10 µl | -80°C | |
4x PRMT5 assay buffer (add DTT before experiment) | 3 ml | -20°C | ||
79556 | Blocking buffer | 50 ml | +4°C | |
HRP chemiluminescent substrate A (transparent bottle) | 6 ml | +4°C | ||
HRP chemiluminescent substrate B (brown bottle) | 6 ml | +4°C | ||
96-well plate precoated with histone substrate | 1 | +4°C |
*The concentration of PRMT5/MEP50 is lot-specific and will be indicated on the tube containing the enzyme.