METTL3/METTL14 Complex Chemiluminescent Assay Kit
The METTL3/METTL14 Complex Chemiluminescent Assay Kit is designed to measure METTL3/METTL14 complex activity for screening and profiling applications. The key to the METTL3/METTL14 Complex Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes N6-methylated adenosine. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Need us to run inhibitor screens or profile your compounds against METTL3/METTL14 complex? Check out our Methyltransferase Screening Service.
TBST buffer (1 x Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween-20)
Dithiothreitol (0.5 M DTT)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform
Catalog # | Component | Amount | Storage | |
100105 | METTL3/METTL14 human enzyme | 20 μg | -80°C | Avoid freeze/ thaw cycles! |
52120 | 20 μM S-adenosylmethionine | 250 μl | -80°C | |
52140Z4 | Primary antibody 29 | 100 μl | -80°C | |
52131H | Secondary HRP-labeled antibody 2 | 10 μl | -80°C | |
78368 | 4x METTL assay buffer* | 3 ml | -20°C | |
Blocking buffer 5 | 50 ml | +4°C | ||
79670 |
ELISA ECL substrate A (translucent bottle) | 6 ml | Room Temp. | |
ELISA ECL substrate B (brown bottle) | 6 ml | Room Temp. | ||
White microplate precoated with the RNA substrate** | 1 | +4°C |
*Important: Use RNAse-free conditions!
**Add 30 μl of 0.5 M DTT.
Liu, J., et al. Nat Chem Biol. 2014; 10(2): 93–95.