c-Raf Kinase Assay Kit

Catalog #: 79570
Size: 96 rxns
$495
Description:
c-Raf (Raf-1; proto-oncogen c-Raf) is a member of the Raf (rapidly accelerated fibrosarcoma) kinase family that plays an important role in regulating the MEK and ERK pathways. The c-Raf Kinase Assay Kit is designed for measuring c-Raf activity for screening and profiling applications. The c-Raf Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant c-Raf enzyme, c-Raf substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Materials Required But Not Provided:

Kinase-Glo MAX (Promega #V6071)

Dithiothreitol (DTT, 1 M; optional)

Microplate reader capable of reading luminescence

Adjustable micropipettor and sterile tips

30°C incubator

Supplied As:
The c-Raf Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant c-Raf enzyme, c-Raf substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Format:

Storage / Stability:
Up to 6 months when stored as recommended.
Application(s):
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
Nakamura, J.L. Expert Opin. Ther. Targets 11(4):463-472 (2007)
Scientific Category:
Kinase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

79570.pdf