JAK3 Assay Service
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Target
JAK3
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Description
Screening and/or profiling inhibitor compounds against JAK3 kinase activity in a biochemical assay.
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Synonyms
Tyrosine-protein kinase JAK3, JAK-3, Leukocyte janus kinase (L-JAK)
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Example Data
*Example only, final data may vary.
Assay Details
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Assay Format
Luminescent
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Reference Compounds and IC50
Staurosporine, 2 nM
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Assay Principle
The JAK3 Assay is designed to measure JAK3 activity for screening and profiling applications using Kinase-Glo® as a detection reagent, which uses ATP-dependent luciferase to measure available ATP in solution. As kinase activity increases, the available ATP in solution decreases, resulting in a corresponding lower luminescent signal. The JAK3 Assay Kit is performed in a 96-well format, using purified recombinant JAK3 enzyme, JAK3 substrate peptide (Poly(Glu;Tyr, 4:1), ATP, and kinase assay buffer.
Target Details
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Protein Family
Kinases
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UniProt
P52333
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Background
Janus kinase (JAK) is a family of intracellular, nonreceptor tyrosine kinases that play an important role in cytokine-mediated signaling. This family includes four protein kinases; JAK1, JAK2, JAK3 and Tyk2. Various studies have suggested that regulation of JAK activity should be a promising approach to combat numerous diseases such as immune deficiencies and cancers.
Delivery
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Estimated Turnaround
Two to three weeks following delivery of compounds
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Results
Extensive report with raw and analyzed data, graphs, and detailed protocols. Includes positive control for inhibition.
