JAK2 Assay Service
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Target
JAK2
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Description
Screening and/or profiling inhibitor compounds against JAK2 kinase activity in a biochemical assay.
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Synonyms
Tyrosine-protein kinase JAK2, JAK-2
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Example Data
*Example only, final data may vary.
Assay Details
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Assay Format
Luminescent
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Reference Compounds and IC50
Staurosporine, 2 nM
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Assay Principle
The JAK2 Assay is designed to measure JAK2 activity for screening and profiling applications using Kinase-Glo® as a detection reagent, which uses ATP-dependent luciferase to measure available ATP in solution. As kinase activity increases, the available ATP in solution decreases, resulting in a corresponding lower luminescent signal. The JAK2 Assay Kit is performed in a 96-well format, using purified recombinant JAK2 enzyme, JAK2 substrate peptide (Poly(Glu;Tyr, 4:1), ATP, and kinase assay buffer.
Target Details
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Protein Family
Kinases
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UniProt
O60674
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Background
Janus kinases (JAKs) are a family of intracellular nonreceptor tyrosine kinases including JAK1, JAK2, JAK3 and Tyk2, that has been recognized as an important modulator in inflammatory processes.
Delivery
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Estimated Turnaround
Two to three weeks following delivery of compounds
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Results
Extensive report with raw and analyzed data, graphs, and detailed protocols. Includes positive control for inhibition.
