HDAC6 Assay Service
●
Target
HDAC6
●
Description
Screening and/or profiling inhibitor compounds against HDAC6 deacetylase activity in a biochemical assay.
●
Synonyms
histone deacetylase, HDAC-6
●
Example Data
*Example only, final data may vary.
Assay Details
●
Assay Format
Fluorogenic
●
Reference Compounds and IC50
TSA, 3 nM; SAHA, 10 nM
●
Assay Principle
The Fluorogenic HDAC6 Assay is a complete assay system designed to measure histone deacetylase 6 (HDAC6) activity for screening and profiling applications. The Fluorogenic HDAC6 Assay is based on a unique fluorogenic substrate and developer combination. This assay uses only two simple steps on a microtiter plate to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.
Target Details
●
Protein Family
Deacetylases
●
UniProt
Q9UBN7
●
Background
HDAC6 is a distinct member of the histone deacetylase family, primarily found in the cytoplasm. Unlike other HDACs it targets non-histone proteins. HDAC6 regulates cell motility by deacetylating α-tubulin and helps degrade misfolded proteins through autophagy. Additionally, it influences stress responses and immune signaling by modulating acetylation of key regulatory proteins. Due to its versatile roles, HDAC6 is a promising drug target with potential therapeutic applications in cancer, neurodegenerative diseases, and immune disorders.
Delivery
●
Estimated Turnaround
Two to three weeks following delivery of compounds
●
Results
Extensive report with raw and analyzed data, graphs, and detailed protocols. Includes positive control for inhibition.
