HDAC1 Assay Service
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Target
HDAC1
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Description
Screening and/or profiling inhibitor compounds against HDAC1 deacetylase activity in a biochemical assay.
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Synonyms
histone deacteylase, HDAC1
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Example Data
*Example only, final data may vary.
Assay Details
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Assay Format
Fluorogenic
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Reference Compounds and IC50
Trichostatin A, 1 nM
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Assay Principle
The Fluorogenic HDAC1 Assay is a complete assay system designed to measure histone deacetylase 1 (HDAC1) activity for screening and profiling applications. The assay includes purified HDAC1 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative control. The HDAC1 assay is based on a unique fluorogenic substrate and developer combination. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC1. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore is then measured using a fluorescence reader.
Target Details
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Protein Family
Deacetylases
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UniProt
Q13547
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Background
HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from acetyllysines in modified proteins. In the HDAC assay, fluorescent-dye molecules are attached to a peptide containing acetyllysine. Attachment to the peptide quenches the fluorescence of the dye. After treatment of the peptide with an HDAC, the reaction is mixed with a development solution that is specific for nonacetylated lysines. If the acetyl group has been removed from the lysine by the HDAC, this solution will release the dye allowing for fluorescence. Fluorescence is therefore directly related to HDAC activity.
Delivery
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Estimated Turnaround
Two to three weeks following delivery of compounds
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Results
Extensive report with raw and analyzed data, graphs, and detailed protocols. Includes positive control for inhibition.
