GSK3β Assay Service
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Target
GSK3β
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Description
Screening and/or profiling inhibitor compounds against GSK3β kinase activity in a biochemical assay.
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Synonyms
Glycogen Synthase Kinase 3 Beta, GSK-3 Beta, Serine/Threonine-Protein Kinase GSK3B
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Example Data
*Example only, final data may vary.
Assay Details
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Assay Format
Luminescent
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Reference Compounds and IC50
CHIR-99021, 7 nM
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Assay Principle
The GSK3β Assay is designed to measure GSK3β activity for screening and profiling applications using Kinase-Glo® as a detection reagent, which uses ATP-dependent luciferase to measure available ATP in solution. As kinase activity increases, the available ATP in solution decreases, resulting in a corresponding lower luminescent signal. The GSK3β Assay Kit is performed in a 96-well format, using purified recombinant GSK3β enzyme, GSK3β substrate (GSK substrate peptide), ATP, and kinase assay buffer.
Target Details
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Protein Family
Kinases
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UniProt
P49841
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Background
GSK3 (Glycogen synthase kinase) is a serine/threonine kinase which initially was found to phosphorylate and inactivate glycogen synthase in glycogen biosynthesis. It is an important regulator enzyme in many disease pathogeneses including cancer, immune disorders, metabolic disorders and neurological disorders like Alzheimer’s disease. In fact, GSK3β, one of the isoforms of GSK3, was identified as a key regulator in tau-driven Alzheimer’s disease.
Delivery
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Estimated Turnaround
Two to three weeks following delivery of compounds
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Results
Extensive report with raw and analyzed data, graphs, and detailed protocols. Includes positive control for inhibition.
