FTO Assay Service
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Target
FTO
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Description
Screening and/or profiling inhibitor compounds against FTO demethylase activity in a biochemical assay.
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Synonyms
ALKBH9, BMIQ14
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Example Data
*Example only, final data may vary.
Assay Details
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Assay Format
Chemiluminescent
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Reference Compounds and IC50
2,4-pyridinedicarboxylic acid, 5.4 μM
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Assay Principle
The FTO Chemiluminescent Assay is designed to measure FTO activity for screening and profiling applications. The key to the FTO Chemiluminescent Assay is a highly specific antibody that recognizes methylated substrate. Signal is inversely related to the activity. Three simple steps on a microtiter plate are required for detection of demethylase activity. First, FTO enzyme is incubated with the substrate. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
Target Details
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Protein Family
Demethylases
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UniProt
Q9C0B1
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Background
The methylation of internal adenosines at the N6 position (m6A) in RNA has been shown to play essential roles in various normal and pathologic processes, including various cancers, leukemia, Alzheimer’s disease, and obesity. FTO (Fat mass and obesity-associated protein), also known as ALKBH9 and BMIQ14, is the first m6A demethylase identified.
Delivery
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Estimated Turnaround
Two to three weeks following delivery of compounds
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Results
Extensive report with raw and analyzed data, graphs, and detailed protocols. Includes positive control for inhibition.
