The FBXL10 Homogeneous Assay Kit is designed to measure the activity of the FBXL10 for screening and profiling applications. The key to the FBXL10 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing FBXL10 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Need us to run inhibitor screens or profile your compounds against FBXL10 (KDM2B)? Check out our Demethylase Screening Services.
Kit comes in a convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate, primary antibody, methylation assay buffer, and purified FBXL10 for 96 or 384 enzyme reactions.
FBXL10, also known as KDM2B, JHDM1B, and CXXC2, is a histone lysine demethylase that exhibits demethylation activity toward trimethyl-lysine 4 (H3K4me3) and dimethyl-lysine 36 (H3K36me2) on histone H3.
References
Iwase, S., et al. Cell 2007; 128(6):1077-1088.
Storage/Stability
At least 6 months from date of receipt when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Instructions for Use
See assay protocol for detailed instructions.
Applications
Useful for studying enzyme kinetics and HTS applications.
Shipping Temperature
-80°C
Notes
AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.
Contraindications
Green and blue dyes that absorb light in the AlphaScreen signal
emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen
quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The
presence of culture medium RPMI 1640 at >1% leads to signal reduction due to the presence of
excess biotin and iron in this medium. MEM, which lacks these components, does not affect
AlphaScreen assays.
Warnings
Avoid freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.