FBXL10 (KDM2B) Homogeneous Assay Kit

Catalog #: 50610
Size: 384 reactions
$1,050
Background:
FBXL10, also known as KDM2B, JHDM1B, and CXXC2, is a histone lysine demethylase that exhibits demethylation activity toward trimethyl-lysine 4 (H3K4me3) and dimethyl-lysine 36 (H3K36me2) on histone H3.
Description:
The FBXL10 Homogeneous Assay Kit is designed to measure the activity of the FBXL10 for screening and profiling applications. The key to the FBXL10 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing FBXL10 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Synonym(s):
KDM2B, JHDM1B, CXXC2
Supplied As:
Kit comes in a convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate, primary antibody, methylation assay buffer, and purified FBXL10 for 384 enzyme reactions.
Contraindications:
Green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of culture medium RPMI 1640 at >1% leads to signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.
Format:

COMPONENTS:

Storage / Stability:
At least one year from date of receipt when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Great for studying enzyme kinetics and HTS applications.
Reference(s):
Iwase, S., et al. Cell 2007; 128(6):1077-1088.
Notes:

The buffer in this kit was reformulated in May of 2015 with a different reducing agent to improve assay performance. The old formulation can still be purchased upon special request.

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Demethylase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

50610.pdf