EZH1 Chemiluminescent Assay Kit
The EZH1 Chemiluminescent Assay Kit is designed to measure activity of the EZH1 complex (EZH1/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes. The key to the EZH1 Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for two hours. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
TBST buffer (1x Tris-buffered saline, pH 8.0, containing 0.05% Tween-20)
Luminometer or microplate reader capable of reading chemiluminescence
Rotating or rocker platform
| Catalog # | Component | Amount | Storage | |
| 51007 | EZH1/EED/SUZ12/RbAp48/AEB2 | 200 µg | -80°C | Avoid freeze/ thaw cycles! |
| 52120 | 250 µM S-adenosylmethionine | 4 x 250 ml | -80°C | |
| 52140F | Primary Antibody 6 | 25 µg | -80°C | |
| 52131H | Secondary HRP-labeled Antibody 2 | 20 µg | -80°C | |
| 52170 | 4x HMT Assay Buffer 1 | 2 x 3 ml | -20°C | |
| 79556 | Blocking Buffer 1 | 2 x 50 ml | +4°C | |
| HRP chemiluminescent substrate A (translucent bottle) | 2 x 6 ml | +4°C | ||
| HRP chemiluminescent substrate B (brown bottle) | 2 x 6 ml | +4°C | ||
| 79961 | 384-well plate precoated with histone substrate | 1 | +4°C | |
1. Dillon, S.C., et al. Genome Biology 2005; 6:227.
2. Morin, R.D., et al. Nat Genet. 2010; 42(2):181.
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