EZH1 Chemiluminescent Assay Kit

Catalog #: 52990
Size: 384 reactions
Species: Human
The EZH1 Chemiluminescent Assay Kit is designed to measure activity of the EZH1 complex (EZH1/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes. The key to the EZH1 Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for two hours. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Materials Required But Not Provided:

TBST buffer (1x Tris-buffered saline, pH 8.0, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Rotating or rocker platform

Supplied As:
The EZH1 Chemiluminescent Assay Kit comes in a convenient format, with wells precoated with histone H3 peptide substrate, an antibody against methylated K27 residue of Histone H3, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase

Instructions for use:
See product data sheet for detailed protocol.
Storage / Stability:
One year from date of receipt when stored as directed.
Great for studying enzyme kinetics and HTS applications.
1. Dillon, S.C., et al.  Genome Biology 2005; 6:227.
2. Morin, R.D., et al. Nat Genet. 2010; 42(2):181.
Avoid freeze/thaw cycles.
Scientific Category:
Product Type:
Assay Kit
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