EGFR (T790M/C797S/ L858R) Assay Service
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Target
EGFR (T790M/C797S/ L858R)
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Description
Screening and/or profiling inhibitor compounds against EGFR (T790M/C797S/ L858R) kinase activity in a biochemical assay.
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Synonyms
EGFR, Epidermal Growth Factor Receptor, ERBB, ErbB-1, HER1, MENA
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Example Data
*Example only, final data may vary.
Assay Details
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Assay Format
Luminescent
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Reference Compounds and IC50
AZD-9291, 0.1 μM
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Assay Principle
The EGFR(T790M/C797S/L858R) Kinase Assay is designed to identify mutant-selective EGFR kinase inhibitors and to measure EGFR (T790M/C797S/L858R) kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
Target Details
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Protein Family
Kinases
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UniProt
P00533
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Background
The epidermal growth factor receptor (EGFR; ErbB-1; HER1) is the cellsurface receptor for members of the epidermal growth factor family. Overexpression and/or hyperactivation of EGFR kinase is associated with several human cancers such as lung, glioblastoma, and epithelian tumors of the neck and head, leading to the development of anticancer therapeutics targeting EGFR. T790M, L858R and C797S mutations in EGFR cause resistance to known EGFR inhibitors.
Delivery
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Estimated Turnaround
Two to three weeks following delivery of compounds
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Results
Extensive report with raw and analyzed data, graphs, and detailed protocols. Includes positive control for inhibition.
