Dicer, His-Tag (Human) Recombinant

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Catalog #

79083

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Description

Recombinant human Dicer (Human Dicer, also known as Dicer 1, Ribonuclease III), full length, encompassing amino acids 2- 1922(end). This construct contains an N-terminal His-Tag (6xHis). This recombinant protein was affinity purified.

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Synonyms

Dicer 1, Ribonuclease III

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Dicer inhibition curve using a Fluorogenic assay (#78855). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

Dicer (His-2-1922(end))

Host Species/Expression System

Sf9

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, and variable imidazole.

221 kDa

Amino Acids

2-1922(end)

Genbank #

NM_030621

UniProt #

Q9UPY3

Tag(s)

N-terminal His-Tag

References

1. Hutvágner,, G., et al. Science. 2001; 293(5531): 834-838.
2. Bernstein, E., et al., Nat. Genetics. 2003; 35: 215-217 (2003).

Application References:
1. A structure-specific small molecule inhibits a miRNA-200 family member precursor and reverses a type 2 diabetes phenotype (2021)

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Datasheets/Protocols

79083

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Dicer, His-Tag (Human) Recombinant

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