Demethylase(s)

 

Demethylases are enzymes that remove methyl groups from lysine and arginine residues of proteins, the most commonly studied being histone demethylases. Methylation of histones is essential for regulating transcription and maintaining chromatin structure, and demethylases, like all histone modifying enzymes, play an important role in development and gene regulation. All of the demethylases, with the exception of LSD1, contain a catalytic jumonji domain. Many of these enzymes require a cofactor such as FAD, Fe(II), or α-ketoglutarate.

Like, methyltransferases, demethylases exhibit a high level of substrate specificity with respect to their target lysine. In addition, they are sensitive to the specific type of methylation that they recognize and subsequently demethylate. For example, some demethylases are only capable of demethylating mono- and dimethyl substrates, whereas others are capable of demethylating all states of lysine methylation.

Given the critical role that demethylases play in gene regulation, it is not surprising that they have been linked to many human cancers. BPS was the first company to supply active, high purity histone demethylases for life science research. We provide a unique and comprehensive offering of enzymes, assay kits, and inhibitors/activators to meet all of your research needs. Our large portfolio of substrates, which includes and a number of modified histones, makes it easy to study histone demethylase substrate specificity.

References:

1.  Pedersen, M.T., et al., Trends Cell Biol. 2010; 20(11): 662-671.

 

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Catalog#SizeProduct NamePrice
50120 20 µg FBXL10 (KDM2B, JHDM1B), FLAG-tag
$460
50156 20 µg FBXL11 (KDM2A, JHDM1A), FLAG-Avi-tags
$460
79344 96 reactions FTO Chemiluminescent Assay Kit
$640
79306 20 µg FTO, His-Tag (E. coli derived)
$450
79480 20 µg FTO, His-Tag (Sf9 derived)
$450
27627-1 10 mg GSK J4 HCl
$170
27627-2 50 mg GSK J4 HCl
$430
50158 500 μl H3K4me2 Substrate
$225
52081 100 µg Histone H3 K27cMe1, Full Length, His-tag
$425
52082 100 µg Histone H3 K27cMe2, Full Length, His-tag
$425
52083 100 µg Histone H3 K27cMe3, Full Length, His-tag
$425
50351 500 µl Histone H3 peptide (1-21), Dimethyl K9, Biotin-labeled
$235
50353 500 µl Histone H3 peptide (1-21), Monomethyl K9, Biotin-labeled
$235
50350 500 µl Histone H3 peptide (1-21), Trimethyl K9, Biotin-labeled
$235
52086 50 µg Histone H3-H4 Tetramer H3K27cMe2, His-tag
$425
52087 50 µg Histone H3-H4 Tetramer H3k27cMe3, His-tag
$425
52090 50 µg Histone Octamer H3K27cMe2, His-tag
$425
52091 50 µg Histone Octamer H3K27cMe3, His-tag
$425
50513 96 reactions JARID1A (KDM5A) Chemiluminescent Assay Kit
$660
50110 20 µg JARID1A (KDM5A), FLAG-tag
$460
50155 20 µg JARID1A (KDM5A), His-Avi-tags, FLAG-tag
$460
50517 96 reactions JARID1B (KDM5B) Chemiluminescent Assay Kit
$680
50514 384 reactions JARID1B (KDM5B) TR-FRET Assay Kit
$1,995
50128 20 µg JARID1B (KDM5B), Full Length, FLAG-tag
$435
50121 20 µg JARID1B (KDM5B, PLU-1), His-FLAG-tags
$450

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