Demethylase(s)

 

Demethylases are enzymes that remove methyl groups from lysine and arginine residues of proteins, the most commonly studied being histone demethylases. Methylation of histones is essential for regulating transcription and maintaining chromatin structure, and demethylases, like all histone modifying enzymes, play an important role in development and gene regulation. All of the demethylases, with the exception of LSD1, contain a catalytic jumonji domain. Many of these enzymes require a cofactor such as FAD, Fe(II), or α-ketoglutarate.

Like, methyltransferases, demethylases exhibit a high level of substrate specificity with respect to their target lysine. In addition, they are sensitive to the specific type of methylation that they recognize and subsequently demethylate. For example, some demethylases are only capable of demethylating mono- and dimethyl substrates, whereas others are capable of demethylating all states of lysine methylation.

Given the critical role that demethylases play in gene regulation, it is not surprising that they have been linked to many human cancers. BPS was the first company to supply active, high purity histone demethylases for life science research. We provide a unique and comprehensive offering of enzymes, assay kits, and inhibitors/activators to meet all of your research needs. Our large portfolio of substrates, which includes and a number of modified histones, makes it easy to study histone demethylase substrate specificity.

References:

1.  Pedersen, M.T., et al., Trends Cell Biol. 2010; 20(11): 662-671.

 

Items 26 to 50 of 99 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4

Catalog#SizeProduct NamePrice
50158 500 μl H3K4me2 Substrate
$230
52081 100 µg Histone H3 K27cMe1, Full Length, His-tag
$440
52082 100 µg Histone H3 K27cMe2, Full Length, His-tag
$440
52083 100 µg Histone H3 K27cMe3, Full Length, His-tag
$440
50351 500 µl Histone H3 peptide (1-21), Dimethyl K9, Biotin-labeled
$240
50353 500 µl Histone H3 peptide (1-21), Monomethyl K9, Biotin-labeled
$240
50350 500 µl Histone H3 peptide (1-21), Trimethyl K9, Biotin-labeled
$240
52086 50 µg Histone H3-H4 Tetramer H3K27cMe2, His-tag
$440
52087 50 µg Histone H3-H4 Tetramer H3k27cMe3, His-tag
$440
52090 50 µg Histone Octamer H3K27cMe2, His-tag
$440
52091 50 µg Histone Octamer H3K27cMe3, His-tag
$440
50513 96 reactions JARID1A (KDM5A) Chemiluminescent Assay Kit
$660
50510 384 reactions JARID1A (KDM5A) Homogeneous Assay Kit
$2,010
50110 20 µg JARID1A (KDM5A), FLAG-tag
$475
50155 20 µg JARID1A (KDM5A), His-Avi-tags, FLAG-tag
$475
50517 96 reactions JARID1B (KDM5B) Chemiluminescent Assay Kit
$700
50512 384 reactions JARID1B (KDM5B) Homogeneous Assay Kit
$2,010
50514 384 reactions JARID1B (KDM5B) TR-FRET Assay Kit
$2,055
50128 20 µg JARID1B (KDM5B), Full Length, FLAG-tag
$450
50121 20 µg JARID1B (KDM5B, PLU-1), His-FLAG-tags
$465
50511 384 reactions JARID1C (KDM5C) Homogeneous Assay Kit
$2,010
50112 20 µg JARID1C(KDM5C), FLAG-tag
$475
50612 96 reactions JHDM1D (KDM7A) Chemiluminescent Assay Kit
$700
50420 384 reactions JHDM1D (KDM7A) Homogeneous Assay Kit
$1,775
50419 20 µg JHDM1D (KDM7A), FLAG-tag
$450

Items 26 to 50 of 99 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4