DDX58, FLAG-Tag Recombinant

Catalog #

100070

$320 *

Size: 100 μg

Qty

Bulk/Custom

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Description

Recombinant human DDX58 (DEAD box protein 58), full length encompassing amino acids 2-925 (end). This construct contains an N-terminal FLAG-Tag. The protein was affinity purified.

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Synonyms

DDX58, DDX58 Protein, DEAD box protein 58, retinoic acid inducible gene I, RIG-1, Antiviral innate immune response receptor RIG-I, Retinoic acid-inducible gene 1 protein, RIG1, RIGI, RIG-I, RIG-I-like receptor 1, RLR-1, SGMRT2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations

Species

Human

Construct

DDX58 (FLAG-2-925(end))

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 100 µg/ml FLAG peptide, 20% glycerol, 3 mM DTT

108 kDa

Amino Acids

2-925(end)

Genbank #

NM_014314

UniProt #

O95786

Tag(s)

N-terminal FLAG-tag

Background

DDX58 (DexD/H-Box-Helicase 58), also known as RIG-I (retinoic acid-inducible gene I) is a crucial protein from the innate immune system, involved in detecting cells infected with viruses such as West Nile Virus, Sendai, Influenza B and Coronaviruses. It recognizes dsRNA (double-stranded RNA) in the cytosol of cells, that have a blunt 5’end, 5’di or tri-phosphate. Once bound to dsRNA, its CARD (caspase activation and recruitment domain) associated with AMVS (mitochondria antiviral signaling proteins) can activate IFN-1 (type 1 Interferon). In addition to recognizing viral RNA, DDX58 also participates in the development of breast cancer cells resistance to treatments by recognizing “self” RNA. Further studies into this interesting protein can reveal new mechanisms of action and be explored for therapeutical applications.

References

1. Jang, M., et al., Cell. 2015; 96(2): 266274.

2. Suarez-Calvet, X., et al., Journal of Pathology. 2014; 233(3): 258-268.

Application Reference(s):

1. Toscana virus non-structural protein NSs acts as E3 ubiquitin ligase promoting RIG-I degradation (2019)