CRISPRa (SAM) HeLa Cell Line

Catalog #

78193

$2,500 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

This cell line has been engineered for use with the CRISPR Synergistic Activation Mediator (SAM) system to induce transcriptional activation and expression of any gene of interest. Cells stably express a mutated dCas9 (Streptococcus pyogenes CRISPR associated protein 9), lacking any endonuclease activity, fused to transcriptional activator VP64. Stable dCas9-VP64 expression is maintained with Blasticidin resistance. Cells also stably express P65 (Transcription Factor p65, or Nuclear Factor NF-κB p65) and HSF1 (Heat Shock Factor 1) fused with an MS2 tag, which is maintained with Hygromycin resistance. When these cells are transfected with an MS2- tagged sgRNA targeting the promoter region of the gene of interest, dCas9-VP64 and MS2-P65-HSF1 are recruited to the genomic DNA and begin transcription, inducing expression of the desired gene.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Induction of PD-1 in CRISPRa (SAM) HeLa cells

Product Info

Storage and Usage

Citations

Host Cell Line

HeLa

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of 10% DMSO

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1S	BPS Bioscience #78221

Mycoplasma Testing

This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.

References

Konermann, S., et al. Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2015. 517(7536): 583-588.

Storage/Stability

Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt.

Culture Conditions

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Growth Medium 1S (BPS Bioscience #78221): MEM medium supplemented with 10% FBS 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin, 200 µg/ml of Hygromycin B and 5 µg/ml Blasticidin to ensure recombinant expression.

Instructions for Use

See assay protocol for detailed instructions.

Applications

1. Quickly generating CRISPR-activated cell pools or cell lines in HeLa cells
2. Implementing sgRNA CRISPRa screens in HeLa cells

Shipping Temperature

-80°C

Notes

The CRISPR/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and 8,771,945, as well as corresponding foreign patents applications, and patent rights.

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
For all questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

CRISPR

Regulatory Status

Research Use Only

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CRISPRa (SAM) HeLa Cell Line

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