CDC7/DBF4 Complex Recombinant

Catalog #

100538

$465 *

Size: 10 µg

Qty

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Description

CDC7/DBF4 complex of human CDC7, also known as HsCDC7 and Hsk 1, GenBank Accession No. NM_003503 a.a. 1-574(end) with N-terminal GST-tag and MW= 94 kDa, and human DBF4, also known as ASK and CHIF, GenBank Accession No. NM_006716, a.a. 1-674(end) with N-terminal GST-tag and MW= 125 kDa, co-expressed expressed in an Sf9 infected baculovirus expression system.

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Synonyms

CDC7: HsCDC7, Hsk 1. DBF4: ASK, CHIF

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 infected baculovirus

Purity

≥90%

Formulation

50 mM Tris-HCL, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF and 25% glycerol.

CDC7: 94 kDa
DBF4: 125 kDa

Amino Acids

CDC7: 1-574(end), DBF4 1-674(end)

Specific Activity

≥8 pmol/min/µg

Genbank #

CDC7: NM_003503
DBF4: NM_006716

UniProt #

CDC7: O00311; DBF4: Q9UBU7

Tag(s)

CDC7: N-terminal GST-tag
DBF4: N-terminal GST-tag

References

1. Hess, G. F. et. al. Gene 211: 133-140, 1998
2. Sheu, Y.-J et. al. Nature 463: 113-117, 2010

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 50 ng/ml BSA, 2 mM ATP, 200 µg of PDKtide synthetic peptide substrate. Add [33P]-ATP and incubate at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Serine-Threonine Kinase

Regulatory Status

Research Use Only