Anti-CD123 Antibody, PE-Labeled (Discontinued)

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Catalog #

101674

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Description

This anti-CD123 antibody is a purified recombinant antibody, which is labeled with R-Phycoerythrin (PE). This antibody has been tested in flow cytometry assay for specific binding to human CD123 on the surface of CD123 CHO Recombinant Cell Line (BPS Bioscience #79640).

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Synonyms

Cell division cycle protein 123 homolog, Protein D123, HT-1080, PZ32, CDC123, C10orf7, D123

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Product Data Gallery

Cell surface expression of CD123 in CD123 CHO Cell Line

Product Info

Storage and Usage

Citations

Species

Human

Isotype

IgG1

Clonality

Monoclonal

Host Species/Expression System

HEK293

Purity

≥90%

Purification

Protein A affinity chromatography from HEK293 supernatants

Format

Aqueous buffer solution

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain protein stabilizer.

Concentration

The concentration will vary with each lot; the exact value will be provided with each shipment.

UniProt #

O75794

Label

PE is an oligomeric protein complex (270 kDa) from red algae that exhibits intensely bright red-orange fluorescence with excitation peak at 566 nm and an emission peak at 574 nm. The complex consists of six heterodimers, α subunit (18 kDa) and β-subunit (20 kDa), and an additional γ-subunit (34 kDa).

Storage/Stability

At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.

Assay Conditions

One million CD123 CHO recombinant cells (BPS Bioscience #79640) were blocked and stained with 1 µg of anti-CD123 antibody, PE-labeled (BPS Bioscience #101674) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. CD123 CHO recombinant cells (green) were compared to parental CHO-K1 cells (blue). Y-axis is the cell count. X-axis is PE intensity.

Applications

Useful for studying expression of CD123 on the cell surface in flow cytometry assay. For flow cytometric staining, the suggested use of this reagent is 5 µl (1 µg antibody) per million cells in 100 µl staining volume.

Shipping Temperature

+4°C (gel packs)

Warnings

Do not freeze

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Anti-CD123 Antibody, PE-Labeled (Discontinued)

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