The StemBright™ Luciferase iPS Cell Pool constitutively expresses the firefly luciferase gene under the control of a CMV promoter, introduced via lentiviral transduction (Firefly Luciferase Lentivirus, BPS Bioscience #79692). Luciferase activity can be used to monitor cell viability, both in vitro and in vivo.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
●
Synonyms
iPSC
●
Product Data Gallery
Oct4 Expression in StemBright™ Luciferase iPS Cell Pool by flow cytometry
Luciferase activity cell titration curve of StemBright™ Luciferase iPS Cell Pool
The cell line has been screened to confirm the absence of Mycoplasma species.
Background
The discovery by Yamanaka and colleagues in 2007 that 4 factors were sufficient to reprogram terminally differentiated fibroblasts into pluripotent stem cells launched the advent of human induced pluripotent stem (iPS) cell technology. These human iPS cells are capable of both self-renewal and differentiation down all three germline lineages and provide both a tool to model human development and disease in the relevant differentiated human cell types, and a unique opportunity for high throughput drug screening.
References
Cao F, et al., 2006 7:1005-1014.
Lepperhof V, et al., 2014 PLoS One. 9(9):
Li X, et al., 2015 Int J Clin Exp Med. 8(6):8938-8947.
Liu Z, et al., 2013 PLoS One. 8(6):
Nishimura, et al., 2022 Methods in Molecular Biology. 2524:291–297.
Takahashi K, et al., 2007 131(5):861–872.
Storage/Stability
Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
As this is a cell pool and not a cell line, BPS Bioscience cannot guarantee the stability of the genetic modifications over time. Clonal selection can be performed. We recommend freezing cell vials very early on and growing the cells for a limited number
Growth Media
For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.
Media Required for Cell Culture iPSC Thaw Medium: mTeSR™ Plus supplemented with 1% Penicillin/Streptomycin.
Complete iPSC Thaw Medium: mTeSR™ Plus supplemented with 1% Penicillin/Streptomycin and 1 µM Thiazovivin. Note: Thiazovivin is unstable once diluted in the medium. Prepare just enough to proceed with thawing of the cells.
iPSC Growth Medium: mTeSR™ Plus supplemented with 1% Penicillin/Streptomycin and 0.5 µg/ml Puromycin.
iPSC Passage Medium: mTeSR™ Plus supplemented with 1% Penicillin/Streptomycin and 0.5 µg/ml Puromycin and 1 µM Thiazovivin.
2X Freezing Medium: 80% mTeSR™ Plus supplemented with 1% Penicillin/Streptomycin, 1 µM Thiazovivin and 20% DMSO (vol/vol).
Media Used in the Functional Cellular Assay iPSC Thaw Medium: mTeSR™ Plus supplemented with 1% Penicillin/Streptomycin (does NOT contain Thiazovivin).
Applications
In vitro cell viability assays
In vitro co-culture assays
Monitor iPS derived cell localization in vivo using bioluminescent imaging (BLI)
Shipping Temperature
-80°C
Notes
License Disclosure The iPSC technology is protected by several patents, including US patent Nos. 8048999, 8058065, 8129187, 8278104, 8530238, 8900871, 9404124, 9499797, 10519425, and patent pending, for which iPS Academia Japan, Inc. has been granted license rights with a sub-licensable right. The purchase of this cell line grants you a 10-year license to use this cell line in your immediate laboratory for internal research purposes. Commercial use of this cell line is not allowed. Commercial use requires the appropriate license from iPS Academia Japan, Inc. This cell line is for research use only, not for therapeutic or prophylactic use in humans or animals. Use in humans is strictly prohibited. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of the cell line in any way. Modification of this cell line or transfer to another facility of the cells requires a separate license or additional fees; contact [email protected] for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use and does not accept any liability in connection with the handling or use of the cell line. Visit bpsbioscience.com/license for the label license and other key information about this product.
Powered by Bioz
