RdRp (SARS-CoV-2) TR-FRET Assay Kit (Discontinued)
The RdRp (SARS-CoV-2) TR-FRET Assay Kit is designed to measure the activity of the SARS-CoV-2 RNA-dependent RNA Polymerase (RdRp) for screening and profiling applications. RdRp operates as a complex of NSP12, NSP7, and NSP8 proteins. It is a crucial enzyme in the life cycle of coronaviruses and represents one of the most promising druggable targets for SARS-CoV-2. The assay measures the direct incorporation of biotinylated ATP into the double-stranded RNA substrate. The increase in TR-FRET signal is proportional to the amount of ATP incorporated in the RNA. With this homogeneous kit, only two steps are required for RdRp activity detection. First, a test compound is incubated with the enzyme in the reaction mixture. Next, Dye-labeled acceptor and Eu-labeled Antibody are added, followed by reading of the TR-FRET signal.
- Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
- Adjustable micropipettor and sterile tips
| Catalog # | Name | Amount | Storage |
| 100839 | NSP12/NSP7/NSP8 (SARS-CoV-2) Complex* | 100 µg | -80°C |
| Digoxigenin-labeled RNA Duplex | 2 x 4 µl | -80°C | |
| Biotinylated ATP | 2 x 4 µl | -80°C | |
| RNAse inhibitor | 100 µl | -20°C | |
| RdRp assay buffer component 1 (Incomplete Buffer) | 2 x 5 ml | -20°C | |
| RdRp assay buffer component 2 (Incomplete Buffer) | 2 x 20 µl | -20°C | |
| DTT (0.5 M) | 2 x 200 µl | -20°C | |
| Eu-labeled anti-Digoxigenin Antibody | 2 x 4 µl | -80°C | |
| Dye-labeled acceptor | 2 x 10 µl | -20°C | |
| RdRp TR-FRET Detection buffer** | 5 ml | -20°C | |
| 79969 | White, nonbinding, low volume, microtiter plate | 1 | Room Temp |
| Plate sealing film | 1 | Room Temp |
*The initial concentration of enzyme is lot-specific and will be indicated on the tube containing the protein.
**Contains a toxic compound. Use appropriate precautions
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