RdRp/NSP7/NSP8 (SARS-CoV-2) Complex Recombinant (Discontinued)

Catalog #

100839

$495 *

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Description

The RdRp (RNA dependent RNA polymerase) complex is a complex of the three SARS-CoV-2 proteins NSP7, NSP8 and RNA polymerase/NSP12, reconstituted from the affinity-purified recombinant proteins. Recombinant SARS-CoV-2 RNA polymerase/NSP12, full length encompassing amino acids 1-932 (end), contains an N-terminal FLAG-tag. Recombinant SARS-CoV-2 NSP7, full length encompassing amino acids 1-84 (end), contains a C-terminal His-tag (6xHis). Recombinant SARS-CoV-2 NSP8, full length encompassing amino acids 1-198 (end), also contains a C-terminal His-tag (6xHis).

This product has been cited 4 times.

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Synonyms

RdRP, RDR, RNA replicase, SARS-coronavirus RNA polymerase, nsp12, nonstructural protein 8, SARS-CoV-2 NSP8, ncov NSP8, NSP 8, NSP-8, nonstructural protein 7, SARS-CoV-2 NSP7, ncov NSP7, NSP 7, NSP-7

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Product Info

Storage and Usage

Citations4

Species

SARS-CoV-2

Construct

RdRp (FLAG-1-932(end)) / NSP7 (1-84(end)-His) / NSP8 (1-198(end)-His)

Host Species/Expression System

RdRp: HEK293; NSP7: E. coli; NSP8: E. coli

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 4 mM MgCl₂, 1 mM TCEP, and 10% glycerol

RdRp: 108 kDa, NSP7: 10 kDa, NSP8: 23 kDa

Amino Acids

RdRp: 1-932(end), NSP7: 1-84(end), NSP8: 1-198(end)

Genbank #

RdRp: YP_009725307; NSP7: YP_009725303.1; NSP8: YP_009725304.1

Tag(s)

RdRp: N-terminal FLAG-tag, NSP7: C-terminal His-Tag, NSP8: C-terminal His-Tag

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RdRp/NSP7/NSP8 (SARS-CoV-2) Complex Recombinant (Discontinued)

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