Recombinant complex of three SARS-CoV-2 proteins NSP7 (nonstructural protein 7), NSP8 (nonstructural protein 8) and NSP12 (nonstructural protein 12). The NSP8_NSP7 construct contains an N-terminal His-tag (14xHis) followed by a TEV protease target sequence that allows cleavage of His-tag (TEV: tobacco etch virus cysteine protease BPS Bioscience #50308). The recombinant NSP12 construct is full length encompassing amino acids 1-932 (end). These recombinant proteins are co-expressed in an E. coli expression system and affinity purified.
Activity was measured using the RdRp (SARS-CoV-2) TR-FRET Assay Kit (BPS Bioscience #78394) with Nsp8_ Nsp7/Nsp 12 (Sars-CoV-2) complex titrated at various concentrations.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
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