PLK2, GST-tag Recombinant

Catalog #

40034

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human Plk2 also known as SNK, polo-like kinase 2, GenBank Accession No. NM_006622, a.a. full length, with N-terminal GST-tag, expressed in a baculovirus infected Sf9 cell expression system. MW=104 kDa.

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Synonyms

SNK, polo-like kinase 2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥51%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris pH 8, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, and 20% glycerol.

104 kDa

Amino Acids

full length

Specific Activity

≥1.6 pmole/min/μg

Genbank #

NM_006622

UniProt #

Q9NYY3

Tag(s)

N-terminal GST-tag

Background

PLK2 or Polo-like kinase 2 is a serum-inducible kinase that is a member of the Polo family of serine/threonine protein kinases which play an essential role in normal cell division. PLK2 phosphorylates the P4.1-associated protein (CPAP), a human homologue of SAS-4, in procentriole formation during the centrosome cycle. PLK2 may also play a role as a tumor suppressor since it interacts with TSC1 protein which in turn inhibits mTOR signaling, tumor growth and chemosensitivity under hypoxic conditions. Human lung tumor cells deficient in PLK2 grow larger than control tumors, and PLK2 interacts with endogenous TSC1 protein in these tumors.

References

1. Syed, N. et al., Blood. 2006 Jan 1,107(1):250-6.
2. Eckerdt, F. et al., Oncogene. 2005 Jan 10,24(2):267-76.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 1 mM DTT using 0.5 mg/ml Casein substrate and 10 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40034 Lot 130806-G1.pdf

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PLK2, GST-tag Recombinant

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