PLK3, His-Tag Recombinant

Catalog #

40035

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human Polo-like kinase 3, or PLK3, GenBank Accession No. NM_004073, a.a. 58-340 with an N-terminal His-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW=37 kDa.

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Synonyms

CNK, FNK, PRK, polo-like kinase 3

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥68%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol.

36.7 kDa

Amino Acids

58–339

Specific Activity

≥110 pmol/min/μg

Genbank #

NM_004073

UniProt #

Q9H4B4

Tag(s)

N-terminal His-tag

Background

PLK3 or Polo-like kinase 3 is a cytokine-inducible kinase that is a member of the Polo family of serine/threonine protein kinases. PLK3 contains both a catalytic domain and a putative regulatory domain and plays an important role in the regulation of cell cycle progression and tumorigenesis. PLK3 is closely associated with centrosomes in several human cell lines and its localization is dependent on the integrity of microtubules. Hence, PLK3 is also involved in regulating microtubule dynamics and centrosomal function.

References

1. Li, Z., et al., J. Biol. Chem. 280 (17), 16843-16850 (2005).
2.Xie,S., et al., Oncogene 23 (21), 3822-3829 (2004).

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 1 mM DTT using 1 mg/ml Casein substrate and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40035 Lot 140317-G4.pdf

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