NK Cell Expansion Kit

Catalog #

78927

$900 *

Size: 1 Kit

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The NK Cell Expansion Kit is suitable for the ex vivo culture and expansion of human natural killer (NK) cells. It contains engineered Growth-Arrested NK Feeder Cells and NK Cell Basal Medium. The Growth-Arrested NK Feeder Cells are K562 cells engineered to express membrane bound IL-21, and other components, to drive the robust activation and expansion of NK cells. The kit provides enough growth-arrested feeder cells for a starter population of 1 million purified NK cells or PBMC cells.

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Product Data Gallery

Flow cytometry analysis of NK cell populations at different time points during expansion

Human NK cell ex vivo fold expansion at different time points during culture

Flow cytometry analysis of the cytotoxicity profile of expanded NK cells

Luciferase activity-based cytotoxicity profile of expanded NK cells

Product Info

Storage and Usage

Citations

Materials Required But Not Supplied

Name	Ordering Information
Human PBMCs	BPS Bioscience #79059
Human Peripheral Blood NK Cells, Frozen	BPS Bioscience #78798
Anti-NCAM1 Antibody, PE-Labeled	BPS Bioscience #101673
Anti-CD3 Antibody, FITC-Labeled	BPS Bioscience #102008
500 x CFSE	BPS Bioscience #82177
1000x 7-AAD	BPS Bioscience #82178
K562	ATCC #CCL-243
eGFP/Firefly Luciferase K562 Cell Line	BPS Bioscience #78911
eGFP/Firefly Luciferase RS4; 11 Cell Line	BPS Bioscience #78926
Clear-bottom, white 96-well tissue culture-treated plate	Corning #3610
U-bottomed 96-well plate	Corning #3799
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Thaw Medium 2	BPS Bioscience #60184
Luminometer

Format

Catalog #	Name	Amount	Storage
78912	Growth-Arrested NK Feeder Cells	2 million cells/ vial x 5 vials	Liquid Nitrogen
82164	NK Cell Basal Medium	500 ml	4°C
90184	Human Interleukin-2	10 µg	-20°C

Background

NK (natural killer) cells are part of the innate immune system. They function in a histocompatibility complex-independent mode and derive from the hematopoietic lineage. They are the first line of defense against cancer. Expression of marker CD56 correlates with NK cell functionality: the CD56bright subset accounts for about 5% of the population and is less cytotoxic than the CD56dim subset. Cytotoxicity can happen by the release of perforin and granzyme, while activation by KARs (killer activating receptors) leads to release of Fas Ligand, TRAIL (TNF-related apoptosis-inducing ligand) and TNFα (tumor necrosis factor-alpha). In a suppressive tumor microenvironment, NK cells can become inhibited and unable to fight cancer cells. Several clinical trials have focused on using ex vivo generated NK cells alone or in combination with other approaches. NK cells can be generated ex vivo from peripheral blood, umbilical cord blood, iPS cells or immortalized NK cell lines. The ability to generate a number of pure cells high enough for human dosage often requires the use of growth factors such as IL-2 (interleukin 2) or IL-15, and feeder cells. The use of NK cells or CAR (chimeric antigen receptor)-NK cells is an expanding area holding great promise in cancer therapy.

References

Du N., et al., 2021 Cancers (Basel) 13 (16): 4129.

Storage/Stability

Feeder Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. NK Cell Basal Medium is shipped in dry ice and stored at -20°C. After the first thaw, the medium can be stored at 4°C for up to 1 month. Alternatively, aliquot the medium into tubes and store at -20°C. Do not re-freeze aliquots after thawing.

Applications

Culture and expansion of freshly isolated or frozen PBMC-derived NK cells, purified and non-purified.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Warnings

Avoid freeze/thaw cycles

Scientific Category

CAR T-Cell Therapy

Regulatory Status

Research Use Only

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