eGFP/Firefly Luciferase K562 Cell Line

Catalog #

78911

$2,050 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The eGFP/Firefly Luciferase K562 Cell Line are engineered human lymphoblast K562 cells expressing firefly luciferase and enhanced GFP (eGFP) driven by an EF1a promoter. The cells were generated by transduction with Firefly Luciferase-eGFP Lentivirus (BPS Bioscience #78741), which is a SIN (self-inactivating) lentivirus.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

eGFP expression and luciferase activity in the the eGFP/Firefly Luciferase K562 Cell Line.

Luciferase activity-based NK cytotoxicity using eGFP/Luciferase K562 and eGFP/ Luciferase RS4;11 as target cells

Product Info

Storage and Usage

Citations

Host Cell Line

K562, human lymphoblast cell line, suspension.

Supplied As

Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2D	BPS Bioscience #79639

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

The K562 cell line was established from a patient with chronic myelogenous leukemia (CML) in terminal blast crisis. The K562 Cell Line does not express antigens CD19, CD20 or CD22, therefore it makes an excellent negative control in assays that measure specific killing by CAR-T cells targeting B cell antigens CD19, CD20, and CD22. It can also be used as a highly sensitive in vitro target in natural killer assays. The presence of eGFP and luciferase allows for easy assay readouts, making this cell line a convenient choice.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 2 (BPS Bioscience #60184): RPMI-1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 2D (BPS Bioscience #79639): RPMI-1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 200 µg/ml of Hygromycin

Applications

In vitro and in vivo bioluminescence imaging (BLI) and fluorescence imaging.
Use as negative control in CAR-T cell co-culture experiments.
Use as target cells in NK co-culture killing assays

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

CAR T-Cell Therapy

Regulatory Status

Research Use Only

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eGFP/Firefly Luciferase K562 Cell Line

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