eGFP/Firefly Luciferase MM.1S Cell Line

Catalog #

78376

$2,370 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

eGFP/Firefly Luciferase MM.1S Cell Line is a stable MM.1S cell line that expresses a firefly luciferase and enhanced GFP (eGFP) cassette driven by a CMV promoter. The cells were transduced with the integrating Firefly Luciferase-eGFP Lentivirus (BPS Bioscience #79980-G) and cloned by limited dilution to get a monoclonal population. This cell line has been validated by flow cytometry for eGFP expression and luciferase activity.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Luciferase activity in GFP/Firefly Luciferase MM.1S Cell Line

Expression of eGFP in the GFP/Firefly Luciferase MM.1S Cell line.

Product Info

Storage and Usage

Citations

Host Cell Line

MM.1S human B lymphoblasts, suspension

Supplied As

Each vial contains >1 x 10^{6^{cells in 1 ml of Cell Freezing Medium (BPS Bioscience, #79796)}}

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2Q	BPS Bioscience #78380

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

MM.1S cells are multiple myeloma human B lymphoblasts. The parent cell line MM.1 was established from the peripheral blood of a multiple myeloma patient who had become resistant to steroid-based therapy. MM.1S cells are sensitive to dexamethasone.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 2 (BPS Bioscience, #60184): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 2Q (BPS Bioscience, #78380): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 500 µg/ml Geneticin

Applications

Control in cell killing assays.
Suitable as a B-cell target of CAR-T or CAR-NK cells during optimization of CAR design.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only