Firefly Luciferase-eGFP Lentivirus (G418) or (Puromycin)

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79980

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Description

The Firefly Luciferase-eGFP Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. These viruses contain a firefly luciferase and eGFP cassette (Luc-P2A-eGFP) driven by a CMV promoter (Figure 1). Both the luciferase and eGFP are coexpressed under the CMV promoter in the transduced cells, allowing greater flexibility for detection of transduced cells.

Figure 1. Schematic of lenti-vector used to generate the firefly luciferase-eGFP lentivirus

Figure 1. Schematic of lenti-vector used to generate the firefly luciferase-eGFP lentivirus

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Product Info

Storage and Usage

Citations

Species

Replication incompetent HIV

Supplied As

Two vials (500 μl x 2) of Firefly Luciferase-eGFP lentivirus at a titer ≥1 x 10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Materials Required But Not Supplied

• HEK293 growth medium or use Thaw Medium 1 (BPS Bioscience #60187)
• 96-well tissue culture treated, white clear-bottom assay plate (Corning, #3610)
• ONE-Step™ luciferase assay system (BPS Bioscience, #60690)
• Luminometer

Formulation

The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Instructions for Use

See assay protocol for detailed instructions

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Biosafety
None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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Firefly Luciferase-eGFP Lentivirus (G418) or (Puromycin)

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