eGFP/Firefly Luciferase U-87 MG Cell Line

Catalog #

78904

$3,250 *

Size: 2 vials

Qty

Bulk/Custom

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Description

eGFP/Firefly Luciferase U-87 MG Cell Line are engineered human glioblastoma U-87 MG cells expressing firefly luciferase and enhanced GFP (eGFP) driven by an EF1a promoter. The cells were generated by transduction with Firefly Luciferase-eGFP Lentivirus (BPS Bioscience #78741), which is a SIN (self-inactivating) lentivirus.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Panel A. eGFP expression was analyzed in eGFP/Firefly Luciferase U-87 MG cells (green) and parental U-87 MG cells (blue) by flow cytometry. Panel B. Fluorescent microscopy of eGFP/Firefly Luciferase U-87 MG cells. Panel C. Luciferase activity was measured

Product Info

Storage and Usage

Citations

Host Cell Line

U-87 MG, human glioblastoma cell line, adherent.

Supplied As

Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1Q	BPS Bioscience #78096

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

U-87 MG is an epithelial cell line isolated from a malignant glioma and it is commonly used in neuroscience and glioblastoma research. The tumor suppressor gene PTEN (phosphatase and tensin homolog) is deleted in the U-87 MG glioblastoma cell line resulting in hyperactivation of the phosphoinositide 3-kinase (PI3K) pathway, which leads to sustained PI(3,4,5)P3 signaling, and thereby hyperactivation of Akt (protein kinase B) and other effectors. Therefore, PTEN-negative U-87MG glioblastoma cells are widely used to study the modulation of the PI3K/Akt/GSK-3β pathway.

References

Allen M., et al., 2016 Trans. Med. 8(354): 1-4.

Moon S.H., et al., 2013 Int J Oncol. 42(3):921-8.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 1 (BPS Bioscience #60187): MEM supplemented with 10% FBS, 1% non-essential amino acids, 1mM Sodium pyruvate, 1% Penicillin/Streptomycin.

Growth Medium 1Q (BPS Bioscience #78096): MEM supplemented with 10% FBS, 1% non-essential amino acids, 1% sodium pyruvate, 1% Penicillin/Streptomycin plus 1µg/ml Puromycin.

Media Required for Functional Cellular Assay
Thaw Medium 1 (BPS Bioscience #60187): MEM supplemented with 10% FBS, 1% non-essential amino acids, 1mM Sodium pyruvate, 1% Penicillin/Streptomycin.

Applications

Use as target cells in CAR-T or NK co-culture killing assays.
In vitro and in vivo bioluminescence imaging studies.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide

Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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Datasheets/Protocols

78904

SDS

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Certificate of Analysis (CoA)

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eGFP/Firefly Luciferase U-87 MG Cell Line

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