Firefly Luciferase Lentivirus (EF1A Promoter/Geneticin, Hygromycin, or Puromycin)
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Catalog #
78740
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Description
The Firefly Luciferase Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The viruses transduce firefly luciferase under the control of an EF1A promoter (Figure 1).
Figure 1. Schematic of the lenti-vector used to generate the Firefly Luciferase Lentivirus (Geneticin).
Figure 1. Schematic of the lenti-vector used to generate the Firefly Luciferase Lentivirus (Hygromycin).
Figure 1. Schematic of the lenti-vector used to generate the Firefly Luciferase Lentivirus (Puromycin).
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Product Data Gallery
Luciferase activity in transduced HEK293 cells (Geneticin)
Luciferase activity in transduced HEK293 cells (Hygromycin)
Luciferase activity in transduced HEK293 cells (Puromycin)
The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.
Storage/Stability
Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.
Applications
Use as a positive control for transduction.
Optimize transduction assays.
Generate a stable cell line expressing Firefly Luciferase with Geneticin, Hygromycin, or Puromycin
Shipping Temperature
-80°C
Notes
To generate a Firefly Luciferase reporter stable cell line, remove the growth medium 48 hours after transduction and replace it with fresh growth medium containing the appropriate amount of Geneticin, Hygromycin, or Puromycin (as pre-determined from a killing curve) for antibiotics selection of transduced cells. Visit Cell Line FAQs (bpsbioscience.com) “What is a kill curve?” for more information.
Biosafety The lentiviruses are produced with a SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and after integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal and are not present in the lentivirus particle. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS Bioscience recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.
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