MCL1, His-Tag Recombinant

Catalog #

40742

$350 *

Size: 100 µg

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Description

Recombinant human MCL1 (myeloid cell leukemia sequence 1), encompassing amino acids 2-327. This construct contains an N-terminal His-tag (6xHis). The recombinant protein was affinity purified.

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Synonyms

bcl2-L-3, BCL2L3, EAT; Mcl-1, MCL1-ES, mcl1/EAT, MCL1L, MCL1S, TM

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Figure 2: Human MCL-1:MCL-1 Ligand [Biotinylated] Binding

Figure 1: Inhibition of MCL1 Binding

MCL-1 inhibition curve using a TR-FRET assay (#79506). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

MCL1 (His-2-327)

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 20% glycerol, and variable Imidazole

36 kDa

Amino Acids

2–327

Genbank #

NM_021960

UniProt #

Q07820

Tag(s)

N-terminal His-tag

References

1. Kozopas, K., et al., PNAS. 1993; 90(8): 3516-3520.

2. Leu, T.., et al., New England Journal of Medicine. 2013; 368: 107-116.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Figure 1: Inhibition of MCL-1 by A-1210477, was measured using the MCL-1 TR-FRET Assay Kit (BPS Bioscience #79506).

Figure 2: Assay run according to MCL-1 TR-FRET Assay Kit (BPS Bioscience #79506).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Apoptosis

Regulatory Status

Research Use Only

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Datasheets/Protocols

40742 Lot 180214

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